A Novel Variant of Gh_D02G0276 Is Required for Root-Knot Nematode Resistance on Chromosome 14 (D02) in Upland Cotton

Recombinant inbred lines (RILs) derived from a random-mated population of eleven Upland cotton parents, including the root-knot nematode (RKN) resistant germplasm line M-240 RNR, were subjected to low coverage genome sequencing.  RILs having recombinations within the known RKN resistance QTL on chromosome 14 were identified and evaluated for RKN resistance.  A mapping interval of 30 Kb was delimited on Chr.14 that contained four candidate genes.  VIGS (virus induced gene silencing) was employed to identify the gene required for Chr.14-mediated RKN resistance.