Development of Hydrateable, Commercially-Relevant Artificial Cotton Leaves and Assay Architecture for Monitoring Insect Resistance to Bt

Technology will be presented on the development of hydrateable, artificial leaves based on commercially relevant transgenic cotton expressing Bt toxins and the assay architecture to contain these artificial leaves for their presentation to larval Lepidoptera for the purpose of the detection of insect resistance to Bt. Research is presented to show proof of concept. An off the shelf, ready and easy to use commercially available diagnostic assay kit is now possible for the routine monitoring of resistance to insect-resistant cotton for each commercial product being produced by industry.