Field studies were conducted at 8 locations in the Upper Gulf Coast, Brazos River Valley, and Southern High Plains to assess the influence of Chaperone on cotton lint yield. Chaperone is a new product containing the active ingredients sodium pnitrophenolate (0.30%), sodium o-nitrophenolate (0.20%), and sodium 5-nitroguaiacolate (0.01%). Previous research reported in the Beltwide Cotton Conferences Proceedings indicated that Chaperone provided positive yield benefits. The objective of this project was to evaluate effects of Chaperone on cotton lint yield. Chaperone was applied at all locations at the early bloom stage. Treatments included an untreated check, and Chaperone at 5 oz. product/acre. Studies varied in size, depending upon location, but generally were field-scale plots of greater than 0.2 acres. Studies were arranged in a randomized complete block design with four replications. The general linear model in SAS was utilized for statistical analysis. Chaperone was applied with a self propelled sprayer at 10 gallons per acre. Studies were machine harvested and subsamples collected for fiber quality analysis.