The goal of this study is to assess the repeatability of HVI fibrogram measurements across multiple instruments. A set of 191 samples representing a wide range of length variation was selected for this study. The samples were selected from a large population representing various growing conditions, varieties, and locations using the AFIS Upper Quartile Length (UQL) and Short Fiber Content by number (SFCn (%)) as selection criteria. Samples were conditioned at 21±1°C and 65±2% RH for at least 48 hours before testing. Three HVIs at the Fiber and Research Institute (FBRI) were used for this study. 10 fibrograms per sample were acquired on each HVI. Simple linear regressions, descriptive statistics, and t-tests were used for data analysis. The result observed revealed that the unused part of the fibrograms varies from HVI to HVI. It is not surprising as this part of the fibrograms is not calibrated. Therefore, to improve the future use of the fibrogram, a calibration procedure needs to be explored. As expected, the regular HVI outputs (calibrated) do not show differences among HVIs except in rare cases, probably due to the natural within-sample variation.