Field experiments were conducted in 2017 in Henry, Limestone, and Macon counties in Alabama to demonstrate the influence of plant growth regulators on genotypic and environmental interactions. Ten cotton varieties (DP 1518 B2XF, DP 1538 B2XF, DP 1646 B2XF, DG 3445 B2XF, NG 4601 B2XF, PHY 330 W3FE, PHY 444 WRF, ST 6182 GLT) with varying maturities and growth habits were planted in conventional seed beds at all locations. Varying rates of mepiquat chloride were applied at first square, first bloom, and 3 weeks after bloom to simulate mild, moderate, and aggressive plant growth regulator application strategies. Fertility and management strategies were standardized across all locations. Growth parameters, lint yield, and fiber quality were assessed. Data is still being analyzed, however, cotton response to plant growth regulators depends largely on environmental factors such as temperature and rainfall and there is likely an interaction between the genotype of the cultivar, the environment, and application of plant growth regulators.