Expression Profiles of Detoxification Genes in Helicoverpa Zea

High-throughput sequencing was used to characterize the transcriptome (expressed gene repertoire) of the bollworm, Helicoverpa zea. Bollworm neonates (<8 hours old) fed on 50 ppm Cry1Ac (experimental) or water (control) were used to make RNA-Seq transcript libraries for sequencing on Illumina Hiseq2500 platform. A total of 254 gene transcripts involved in detoxification pathways and 62 gene transcripts associated with resistance to Bacillus thuringiensis toxins (Bt toxins) were evaluated to by expression profiling of RNA-Seq data. Transcripts with significant differences in gene expression between control and experimental samples were identified using statistical tests with corrections for multiple testing.