A Simple Low-Cost Method for Detecting and/or Tracking Specific Copy Number Variants (CNV) in Gossypium

Fast and inexpensive methods are needed to discern variations in copy number among non-polymorphic sequence-based markers and genes.  For example, our cytogenetic project needs to screen for and/or verify aneuploids that differ only in chromosome complement from full-sib homozygotes.

We have devised a useful approach that enables the detection of major changes in copy number, i.e., copy number variation (CNV).

This approach has been verified against several known aneuploid lines, but should also be applicable to the tracking of other types of CNV, e.g., due to localized gene duplications, as well as duplications of chromosome segments.