The Effect of Phytohormones to the Dynamics of Protein Biosynthesis and Enzyme Activity In Linted and Naked Seed Cottons

We determined the effect of exogenous indolil-3-acetic acid, á-naphthyl-3-acetic acid and gibberellic acid-3 on enzymatic activity of glucansynthase, peroxidase and cellulase in ovule development of naked and linted cotton (Gossypium hirsutum L.) seeds. We isolated a protein–inhibitor of 37 kDa with pI 4.2 from integument tissue of naked cotton seeds. In addition, we studied its inhibitory activity to the biosynthesis of cellulose after gibberellic acid-3 treatment of ovules of linted cottonseed. The results should be useful for understanding of lint development in cotton. Thus, phytohormones IAA, NAA and GA3 have different effects on protein biosynthesis and alter the activity of glucansynthase, peroxidase and cellulase enzymes active during fiber formation. The study showed an increase in the number and length of fiber in linted cotton under the action of phytohormones. The changes in structure of ovule surface of naked seed were marked, but appearance of fiber was not observed. This suggests that naked seeding of L-70 cotton line is genetically determined. Furthermore, the content of protein-inhibitor with Mr 37 kDa increased in integument tissue of naked seed cotton under the effect of GA3. The research of modulators of cotton fiber development will allow understanding the typical mechanisms of development of fiber with different quality. The knowledge about these properties is discovered aspects for the choice of parental pairs during cotton selection, and control of growth processes.