Non-Destructive Quantification of Oil and Protein in Cottonseed by Time-Domain Nuclear Magnetic Resonance

Modification of cotton seed quality traits is likely to be achieved through a combination of genetic modification, nutrient allocation, and selective breeding. Oil and protein stores comprise the majority of mass of cottonseed embryos. A more comprehensive understanding of the relationship between fiber quality, fiber yield and embryo reserves will help assist breeders in their efforts to improve seed value. Here we report a method for the non-destructive quantification of oil and protein levels within cottonseed by 1H time-domain nuclear magnetic resonance (TD-NMR). This approach is suitable for a minimal amount of seed and represents an accurate, non-invasive alternative to conventional, time consuming chemical extractions.