Use of cpSSR Markers to Identify the Allotetraploid Species Cytoplasms

Sixteen chloroplast microsatellite markers were studied in allotetraploid cotton species, G.hirsutum (AD1), G.barbadense (AD2), G.tomentosum (AD3), G.mustelinum (AD4), and G.darwini( AD5). These species contains the A and the D genomes (AADD; 2n=4=52) and originated from hybridization between an African or an Asian diploid species (genome AA; 2n=26) to an American diploid (genome DD; 2n=26). The markers are used to distinguish these allotetraploid species from each other. Three accessions for each allotetraploid Gossypium species are used in this study.