9524 Evaluation Of Different Methods Of Nuclear Dna Extraction For Lygus hesperus Samples Preserved In Various Storage Conditions

Thursday, January 8, 2009: 11:30 AM
Salon I (Marriott Rivercenter Hotel)
Anup Bastola, Megha N. Parajulee and Ram B. Shrestha, Texas AgriLife Research, Lubbock, TX
The quality and quantity of DNA sample is very important for various molecular biology studies of Lygus. Four common nuclear DNA extraction methods (Phenol chloroform, Chilex, CTAB and Salting out) and three different commercial kits (EZNA Insect DNA kit, Master Pure DNA purification Kit and D`Neasy tissue kit) were evaluated for Lygus specimens preserved in four different storage conditions (dry preserved, alcohol preserved, frozen and fresh). Twenty five adult Lygus samples were used for each method. DNA samples were quantified and quality was assessed by photosphectometry and agarose gel electrophoresis. The applicability of the DNA samples in population genetic study using SSR, ISSR, AFLP were evaluated. Both advantage and disadvantages of different DNA extraction methods will be discussed.
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