Research has led to the discovery that glyphosate binds and complexes certain cations such as iron (Fe), calcium (Ca), and manganese (Mn) when used in tank-mixed solutions.Manganese is a micronutrient required for chlorophyll synthesis and the activation of many enzymes in plant tissue. Therefore, a field experiment was conducted at the Texas Agricultural Experiment Station in Burleson County to evaluate the nutrient concentrations in glyphosate treated cotton. The experiment was designed as a split-plot with two varieties (DP488BR and DP143B2F) as the main plots and two subplots (glyphosate application and no glyphosate application). Each treatment was replicated four times on a Weswood silt loam. At three and ten days after glyphosate application, twenty leaves per plot were collected and analyzed for total nutrient concentration.  Data indicated that the glyphosate application did not significantly impact the nutrient levels in cotton leaves.