We collected cotton gin waste from five cotton gin mills in southeatern Virginia. These materials were characterized form for their summative composition. The materials were subjected to steam treatment at severity parameter of 3.8 using a one ton per hour continuous Stake steam gun. The steam exploded material was hydrolyzed using commercial cellulase enzyme preparation for forty-eight hours at pH 4.7 and 50 oC in a citrate buffer solution. The hydrolysate was fermented using Saccharomyces cerevisiae. All experiments were carried at sixty liter level. The solids loading was 10 wt% for all hydrolysis experiments. In this paper we will report some of our results in this large scale experiments and the potential for commercialization of the process technology.