The root-knot nematode (RKN) is a serious pest of cotton, particularly in RKN-infested fields with coarser textured soils. The detrimental effects of RKN infection on cotton production are more severe in dryland fields during years with insufficient rainfall. Host plant resistance is an effective means of managing RKN infestation, but no commercial variety is highly resistant to RKN despite the availability of germplasm lines with near-immunity. Several cotton varieties with a moderate level of resistance have been available to cotton growers in CA (Acala NemX) and the Mid-South (ST LA887, ST5599BR, PM1560 and PM1560BR), but the later maturity of these varieties reduces their suitability for culture in the northern Mid-South. The purpose of this research was to evaluate several earlier maturity experimental cotton lines developed by Phytogen Seed Company for agronomic performance and RKN resistance in an RKN-infested field in MO.