Spatially variable application of cotton defoliants was evaluated on commercially produced cotton. Evaluations were arranged in a randomized block design with four replicates. Spatially variable treatments were applied using commercially available aerial applicators. Treatments varied spatially based on normalized difference vegetative index values taken of the field two weeks before defoliation application. Treatments were evaluated by comparisons of defoliation cost and lint quality. Lint quality was determined by harvesting at least one cotton module from each plot. Cotton bales from these modules were then classed by the USDA classing office in Rayville, LA. Previous evaluations have indicated that considerable costs savings in defoliation cost can be obtained using spatially variable defoliation applications without significant impact on lint quality.