Unacceptable control of Italian ryegrass with glyphosate has been observed in some populations in the Mississippi delta. Glyphosate-tolerant (GT) Italian ryegrass populations can severely limit the number of effective pre-plant burndown options for minimum and reduced till crop production systems. A series of greenhouse and field studies were conducted near Stoneville, MS to confirm increased tolerance to glyphosate in Italian ryegrass and to develop methods of effective control. In greenhouse studies, glyphosate titration studies were conducted using a known-susceptible (S) population with limited exposure to herbicides and two suspected GT populations collected from producer fields where glyphosate was no longer effective. GT populations were determined to be 3-fold more tolerant to glyphosate than the S population. Increased tolerance to glyphosate in Italian ryegrass will likely limit the use of glyphosate-based spring burndown programs in the Mid-Southern United States. Dicamba or 2,4-D are often mixed with spring-applied glyphosate burndown programs to provide improved control of broadleaf weeds including glyphosate-resistant horseweed (Conyza canadensis). However, the addition of these herbicides to glyphosate have been known to reduce grass control. Therefore the utility of glyphosate-based spring burndown programs may be greatly reduced where GT Italian ryegrass populations are present. Italian ryegrass might be more effectively controlled using fall-applied residual herbicides. Several field studies were conducted at two GT Italian ryegrass sites to determine the feasibility of controlling these populations in cotton and soybean using November-applied residual herbicides.