In order to Introgress elite QTL alleles of G. barbadense L. for fiber quality and resistance to verticillium wilt into G. hirsutum L. and enlarge the narrow genetic base of G. hirsutum and provid more new gerplasm resource for the variety development, the elite G. hirsutum variety �eZhongmiansuo36' and G. barbadense �eHai1' were respectively used as recurrent and donor parent to produce backcross populations, including the 1st (BC1 and BC1S1) and 2nd(BC2F1) backcross generations. QTL analysis of verticillium wilt resistance trait on three phenotypic data sets were proceeded by Composite Interval Mapping. A total of 1123 SSR primer pairs were used to screen polymorphism among two parents Zhongmiansuo36 , Hai1 and their F1 progeny, which resulted in 357 polymorphic primer pairs, and 226 of them were used to screen the BC1F1 population. Linkage test indicated 220 of 239 locus derived from 226 primer pairs could be mapped to 32 linkage groups and covered a total genetic distance of 2408cM, approximately 40% of cotton genome.The average distance of markers were 10.9cM. 26 of all linkage groups were assigned to corresponding chromosome. 23 QTLs for verticillium wilt were mapped in BC2F1 and BC1S1 generations. QTL numbers for disease index and ratio of susceptivity were 17 and 6, respectively. Each QTL could explain 6.4%�`24.5% of phenotypic variance. One QTL for disease index on the linkage LG17 with 0.3cM away from the nearest loci could be detected in BC2F1 from verticillium wilt nursery evaluation and 2 generations of BC2F1 and BC1S1 from the field condition, which could explain 24.5% of phenotypic variance at most, which showed the stable genetic effects for resistance to verticillium wilt. This resistance allele was derived from the sea island cotton parent and could be used for marker assisted breeding. Key words: Upland cotton (G. hirsutum), G. barbadense, AB-QTL, verticillium wilt resistance