Jinfa Zhang1, Wu Wang2, Mingxiong Pang1, R. Esmail1, James Stewart3, and Richard G. Percy4. (1) New Mexico State University, Department of Agronomy and Horticulture, Skeen Hall, Las Cruces, NM 88003, (2) New Mexico State University, Department of Plant and Environmental Sciences, Las Cruces, NM 88003, (3) University of Arkansas, Department of Crop, Soil, and Environmental Sciences, 115 Plant Science Building, Fayetteville, AR 72701, (4) USDA-ARS, 21881 N. Cardon Lane, Maricopa, AZ 85239
This poster presents the results in using AFLP and GT-AFLP markers to evaluate >80 germplasm lines or accessions from five tetraploid cotton species. A gene targeted AFLP (GT-AFLP) system has been tested using primers designed from gene families encoding for transcription factors and the results have proven that clustering using the new marker system is high consistent with that using AFLP analysis. Therefore, GT-AFLP is successful in evaluating genetic diversity among the five species.