John R. Ruberson1, Phillip Roberts1, and J.P. Michaud2. (1) University of Georgia, Dept. of Entomology, Tifton, GA 31793, (2) Kansas State University, 1232 240th Avenue, Agricultural Research Center - Hays, Hays, KS 67601-9228
Preliminary studies of pyrethroid susceptibility in a Georgia population of the lady beetle Hippodamia convergens indicated that at least one population in Georgia had elevated tolerance for pyrethroid insecticides. We expanded the samples in 2006 to include populations from 4 widely-dispersed counties in southern Georgia, and an outlier population of H. convergens from Kansas and a Tift County, Georgia, population of Coccinella septempunctata for comparison. Technical grade material of the pyrethroid l-cyhalothin was used in all bioassays, with the material dissolved in acetone and 1 ul of solution applied directly to the abdominal venter of adult beetles. A range of dosages was tested, and the responses of all of the Georgia populations were comparable to one another. All of the Georgia populations were significantly less susceptible to the pyrethroid than was the Kansas populations, and both the Georgia and Kansas populations were less susceptible than was the C. septempunctata population. The results suggest that many, if not all H. convergens are inherently more tolerant of pyrethroids than at least one population of C. septempunctata, and that the Georgia populations of H. convergens are even more resistant to pyrethroids than conspecifics from the Midwest. The mechanisms for these differences are unknown at present.
Poster (.pdf format, 713.0 kb)