Root characteristics can be important in determining the response of plant to drought. Studies in rice (Oryza sativa L) have shown that root length, number of roots, and root density in in the 20 to 40 cm soil layer were positively correlated with drought tolerance. Therefore genetic modification of rooting system may lead to more drought tolerant cotton. This experiment was designed to evaluate root traits in interspecific Gossypium hirsutum x Gossypium barbadense F2 population. Root length, fresh root weight, dry root weight, lateral root numbers, plant height, and root shoot ratio were measured in green house conditions. The two parents were significantly different for all root traits measured. All measured traits were significantly and positively correlated. QTL mapping for these root traits will be presented.