Historical records indicated that the cultivar ‘Sealand 883' and ‘Sealand 542'were developed through interspecific hybridization with Sea Island cotton (Gossypium barbadense). Recent DNA fingerprinting analysis of over 300 upland cotton (Gossypium hirsutum) lines have indicated the presence of G barbadense introgression in the cultivar ‘Sealand 883' and ‘Sealand 542'. Retention of these introgressed chromatins may suggest the presence of favorable alleles for improving upland cotton. However the extent and effect of these introgressed segments is not fully understood. The objective of the present study are to (1) identify the introgressed G. barbadense chromatin by identifying molecular markers that fall on these segments, and (2) to evaluate the contribution of these chromatins in improving fiber quality traits of Sealand cultivars.