In a previous study, Fourier transform infrared spectroscopy (FTIR) was utilized in identifying different types of botanical cotton trash as each was subjected to simulations of ginning and textile processing. Changes in the infrared spectra that occurred after heat treatment indicated the need to further study the effects of moisture content in the different trash types on resultant FTIR spectra. This study will attempt to determine the effects on different trash types as the levels of moisture content are varied, therefore providing a more in depth investigation in identifying the type of trash present in cotton fibers.