University and private consultant trials conducted in the years 2001 through 2006 located in the major cotton producing areas of the United States are reviewed in this paper. Physiological effects noted in cotton, at over 200 locations, treated with Chaperone™ in comparison with untreated plants during the six year study are chronicled in this study. Physiological effects shown in the paper include studies observing and noting the resulting differences observed in comparison of Chaperone™ treated plants and untreated plants on boll weights, boll numbers, seed weights, seed numbers and lint yield, among others, during the past six years of testing. The resulting physiological effects, directly attributable to the protein enhancement characteristics of Chaperone™ are noted and chronicled in the paper.