Diallel analyses were run on a set of American Pima cultivars and on a set of G. barbadense cultivars of American, Egyptian, Israeli, and Uzbeki origin. General combining ability (GCA) and specific combining ability (SCA) were calculated for cultivars of both sets. Molecular marker data was collected on all parental cultivars and genetic distances calculated. GCA and SCA values were compared between the two data sets. SCA values and genetic distance values were compared to determine if any relationship existed between the two.