Biopolymers are becoming more popular for several medical applications, especially bioactive materials for artificial hips, artificial heart valves, artificial joints and scaffolds that heal and promote growth of tissues. Scaffolds made up of poly (L-lactic acid) or glycolic acid or collagens are few among them. Enzymes may be incorporated into the biopolymer nanofibers so as to make them bioactive. These bioactive nanofibers can promote various biological functions, such as secretion of insulin by pancreas or angiogenesis or cell proliferation etc. Nanofiber scaffold is an emerging area of research with potentially a large number of medical applications. Hyaluronic acid (HA) is one such bioactive material. Hyaluronic acid aids in cellular repair, heals wounds faster as it helps in mitosis of epithelial cells, cell migration, cell proliferation and angiogenesis and so the rate of wound healing is faster. Hence, it was postulated that hyaluronic acid would be a perfect material for making a nanofiber wound dressing. Hyaluronic acid was dissolved in de-ionised water and electrospun. The average diameter of the Hyaluronic acid nanofibers made by electro spinning increased with the increase of flow rate and spin length, whereas the average diameter decreased with the increase in the applied voltage. The air permeability of hyaluronic acid nanofiber dressing was very much higher than Vaseline gauze dressing. Degree of crystallinity of Hyaluronic acid nanofibers was found to be 20.6%. Wound healing of hyaluronic acid nanofiber wound dressing was compared with Band-Aid, solid HA, vaseline gauze and silver dressing in a clinical trial. Based on the analysis of the photographs taken by a digital camera and the pathological report, it was concluded that the healing of the wounds was the best in case of HA nanofiber wound dressing as compared to Band-Aid, Hyaluronic acid powder, Vaseline gauze dressing and the Silver dressing. For the scaffold function HA was mixed with carboxy-methyl cellulose and electro spun to produce a nanoweb that can be used in different cell- growth media to support cell proliferation.