One of the inherent characteristics that makes cotton “king” among fibers and enhances consumers' appeal is its comfort characteristics. More importantly, moisture vapor transport properties of cotton are higher than those of synthetic fibers. It is important to understand the relationship among seed traits, fiber quality and the moisture vapor transport properties (MVTR). This has direct implications on the end-use and marketing of cotton fibers. To the best of our knowledge, such a study involving the relationship between fiber quality and moisture vapor transport of fabrics made from them has not been reported. A preliminary breathability study involving 11 untreated single jersey knitted fabrics spun from 11 different cottons was undertaken. The experimental procedure to evaluate the breathability involves the evaporation of water vapor over time through the fabric and is in accordance with the British Standard BS: 7209:1990. Cottons from three crop years: 2001, 2002 and 2003 from Mississippi and Texas, which include the dominant variety planted in the US over the last three years and varieties varying in breeding methods were selected. These cotton fibers were spun into 20s Ne ring spun yarn using identical spinning conditions and knitted into single jersey fabrics. Since the primary objective of the study was to investigate the effects of fiber quality on the MVTR, it was thought logical to quantify MVTR of fabrics in their griege stage. Preliminary results were interesting and gave good indication that short fiber content and lack of weathering (a low +b) influence the MVTR. A correlation was found between the SFC, +b and MVTR values. The higher is the MVTR value, the better is the breathability and comfort. Based on the preliminary study, fabrics produced from DP 555BG/RR varieties gave the highest MVTR and hence moisture comfort properties. Further trails will be carried out with wide variety of cottons to establish the relationship between fiber quality and comfort.