A one-year study was conducted at the Texas Agricultural Experiment Station in Burleson County, Texas, to examine the physiological effects of Roundup applications on Roundup Ready Flex cotton. This study contained nine different treatments. The treatments were replicated four times in a randomized complete block design on forty inch row spacing. The parameters examined included height and nodal counts, nodes above white flower, box mapping, lint yield and lint quality. All collected data was subjected to the mixed procedure of SAS v9.0. Preliminary analysis indicates significant differences among treatments.