The possibility of obtaining cotton (Gossypium barbadense L. x G. hirsutum L.) haploid, doubled haploid or aneuploid progeny by in vivo and in vitro methods was investigated. F1 interspecific cotton hybrids were pollinated with pollen from Abelmoschus esculentus (okra). Six cross combinations were made between two G. barbadense (Carnak and B403) and three G. hirsutum (Coker 310, Acala Sindos and 4S) commercial Greek varieties and Abelmoschus esculentus ‘Pilea'. From 1020 pollinated flowers, 600 were left on the plant to grow naturally, 240 were collected at 5 and 10 days after pollination (DAP) for in vitro culture, and 180 were harvested at 1, 2 and 3 DAP to monitor okra pollen germination on the cotton stigma and pollen tube behaviour in the style. From the buds left to grow in planta, 22 bolls reached maturity, one or more from each of the six G. barbadense x G. hirsutum hybrids. The bolls contained 67 mature seeds, and eventually produced 40 plants (Pa). The in ovulo culture method yielded 39 young embryos from G. barbadense x G. hirsutum and reciprocal hybrids, and eventually led to 5 Pa plantlets. Fluorescence microscopy of okra pollen tube behaviour on the stigma and in the cotton style revealed that pollen germinated abnormally, and that only a few pollen tubes reached the ovules, and none were observed to enter them. All Pa plants produced from in vivo and in ovulo production methods exhibited morphological traits of both cotton species, were partially fertile, and lacked morphological traits of Abelmoschus esculentus. Root-tip chromosome counts and meiotic Metaphase-I analyses of Pa plants revealed chromosome numbers ranged from 33 to 44. Flow cytometric analysis also indicated Pa plants were aneuploid. The underlying cytological mechanisms remain to be explored, but in the meantime, it may be possible to apply these methods to genome mapping and breeding.