Roller ginning is a slow, expensive, but superior method of ginning cotton. Roller ginning is currently used to gin Pima cotton which comprises only about 3% of the total U.S. cotton crop. Recent developments have demonstrated that much faster roller ginning is possible, making roller ginning feasible for ginning upland cotton. However, current roller gins tend to remove the short linter fibers from fuzzy seed upland cotton, making the lint less desirable. Enabling roller ginning to gin only the longer fibers in combination with much faster ginning rates may open up new markets for a superior-quality fiber. Newly-designed stationary and rotary knives were built and tested. The knives have the potential of discriminating between long and short fibers, ginning the longer fibers and leaving the shorter fibers on the cottonseed. An experiment will determine the performance of the experimental knives and the characteristics of the fiber and cottonseed. Results to be presented include operating parameters of the roller gin stand, HVI and AFIS fiber properties, linters on the cottonseed, and cottonseed grade analysis.