Thursday, 5 January 2006 - 3:30 PM

Development of an Adult Resistance Assay for Monitoring Larval Bt Resistance in the Tobacco Budworm

R. Michael Roe, J. R. Bradley, Jaap VanKretschmar, Christof F. Stumpf, and Kevin V. Donohue. Department of Entomology, North Carolina State University, Box 7647, Raleigh, NC 27695

Current approaches to monitoring Bt resistance to caterpillars involve the evaluation of resistance in lepidopteran larvae using a variety of possible methods, the most common being susceptibility to a single or multiple doses of Bt. Caterpillars needed for resistance monitoring can be obtained directly from the field as larvae or eggs which are allowed to hatch after collection and from mated adult female moths which are allowed to oviposit their eggs after collection. One important problem in any resistance monitoring strategy is the difficulty of obtaining a large enough sample size; this is especially difficult for collecting eggs and larvae. The goal of our research is to investigate methods of monitoring larval resistance to Bt transgenic crops using adult Lepidoptera. We will present different possible strategies and preliminary data on proof of concept. The advantage of using the adult stage is that insects can be collected with phermomone or light traps.

[ Recorded presentation ] Recorded presentation

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See more of The Beltwide Cotton Conferences, January 3-6 2006