A molecular level understanding the mechanism-of-action of pesticides is necessary to define the application strategy appropriate to control pests that prey on row crops. Towards this end we have added standard techniques of single-cell recording from living insect neurons in primary culture to define the activity of Flonicamid, a newly developed pesticidal agent directed against piercing and sucking pests. We found by directly recording ionic current flow through nicotinic ACh channels that Flonicamid failed to alter the flow of ionic current through this channel in the species tested in this work. Our findings directly contradict previous speculation within the commercial sector. We found that Flonicamid is not a neo-nicotinoid. In addition, we have applied the whole-cell recording approach to assess the effects of Flonicamid on voltage-gated ion channels.