Cotton containing dead and/or immature fiber is a major concern for textile dyers of high quality goods. In an un-dyed state, entangled fiber clusters are generically classified as neps. It is only after the application of dye, when some neps remain un-dyed, that the more specific classification of “white speck” is used. Current fiber property measurement systems (HVI) are important in the marketing and general quality assessment of the cotton crop. However, the measurements are not detailed enough to address immature/dead fiber content. The objective of this pilot study was to examine the relationship between the AFIS fiber property distributions and white speck occurrence on dyed yarn. The data was tested for interdependent relationships between key fiber properties such as length and maturity and the occurrence of white specks. Ten bales of cotton were selected with a range of micronaire and tested on Zellweger Uster's 900 A HVI consisted of four color, four micronaire, and 10 length and strength measurements. The 10 samples of each bale were also analyzed using Zellweger Uster's Advanced Fiber Information System (AFIS). After each sample was processed into yarn and dyed, a white speck count comparison, layer-to-layer, was made to determine the amount of variation within a bale utilizing the white speck yarn methodology. AFIS fiber properties such as length, maturity, fineness, and neps have shown a strong relationship with the white specks occurrence. With the combination of low maturity, length, and high nep content, fineness value below 190 millitex would be a good indication for high white speck count. The interdependent relationships between key fiber properties and the occurrence of white specks indicate the possibility of predicting and preventing cotton with high white speck potential from processing.