Cotton (Gossypium Hirsutum L. ) is a major raw crop grown primarily for fibers and seeds. This is man's most important clothing material and widely used in many medical applications. It is typical example for fiber where the porosity arises from the amorphous regions of the structure . For the optimum use of all material, it is very important to know the microstructure of this fiber, the surface energy, the influence of working temperature as well as the temperature of conditioning in these properties. Inverse Gass Chromatography appears to be an appropriate method for the evaluation of the surface characteristics of the cotton fiber. Since Inverse Gas Chromatography is very sensitive to minor variations in surface characteristics.