Today there are many distinct varieties with several different combinations of transgenes. Each transgenic cultivar, depending on the selection process and the number of bags needed for cultivar release, should take around 15 years to develop and release. Using transgenic cultivars in the initial cross of a breeding program could give breeders alternatives to the time scale associated with conventional methods of backcrossing the transgenes into agronomically acceptable cultivars. However, using these parents could reduce the probability of finding a transgressive segregate in the resulting population and limit the application of the final product. Several different scenarios have diverse out comes and possess specific qualities and drawbacks depending on the transgenic composition of each parent. These scenarios and other concerns will be reviewed in accordance to objective 5 of the S-304 “Development of Genetic Resources for Cotton” project.