Allegiance FL / RTU Baytan-Thiram 1.76, Dynasty CST / Systane 40 WP, Terraclor Super X 18.8G and combinations of experimental seed treatments were evaluated for cotton stand establishment under enhanced and natural pathogen populations. Tests were evaluated at the Tennessee Valley Research and Extension Center in Belle Mina and the E.V. Smith Research Center in Tallassee Alabama and the R.R. Foil Plant Science Research Center at Mississippi State University, MS. Plots consisted of four rows 40 ft. long and 38 in. spacing in a randomize complete block design. Two of the four rows were infested, in the furrow at planting time, with a mixture of winter rye grown Rhizoctonia solani and pearl millet grown Pythium ultimum. Blocks were separated by a 20 ft. alley. At 28 days after planting significant treatment differences were found in cotton seedling stand in the enhanced pathogen plots at both Alabama locations but not in the natural pathogen populations. Few differences between treatments were found for seedling stand in the enhanced or natural pathogen plots at the Mississippi State University location. Analyzing seedling stand across locations a significant location, treatment and treatment by location interaction was determined indicating treatment response is influenced by environment and pathogen of a given site.