Ned M. French, Eden Bioscience Corporation, 15200 Burlingame Road, Little Rock, AR 72223-9618, T.L. Kirkpatrick, University of Arkansas, Southwest Research and Extension Center, 362 Highway 174 North, Hope, AR 71801, and Zhongmin Wei, Eden Bioscience, Corp., 11816 North Creek Parkway N., Bothell, WA 98011-8201.
A study was initiated to examine the effects transgenically-expressed harpinEA constructs on cotton infected with nematodes. Each test was conducted by Dr. Terry Kirkpatrick. All plants were grown in a growth room environment. At planting, each pot was inoculated with eggs of root-knot nematode, Meloidogyne incognita. The first set of transgenically-expressed harpinEA constructs (PE58) were evaluated in a growth room experiment conducted in 2003 and 2004. In the subsequent experiment, a new construct source was tested, and three promising constructs were pulled forward from the first experiment. Trials were terminated at approximately 45 days after initiation. Above and below ground plant measurements, root galling, numbers of root knot nematode eggs and juveniles were recorded.
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