A multi-state study comparing the yield effects derived by an application of Chaperone in side by side testing conducted in commercial cotton fields during the 2005 season. Trials were conducted in the states of Texas, New Mexico, Kansas, Oklahoma and Tennessee during the 2005 crop season to determine the probability of beneficial yield effects derived by a single application of the nitro-phenolic compound Chaperone in comparison to untreated check blocks. All studies were in commercial cotton fields with the untreated check and the Chaperone treated cotton placed side by side and with no other changes in agricultural practices than the single application of Chaperone during the blooming period.