Research was initiated in 2005 at the West Florida Research and Education Center in Jay, FL. Three tillage regimes were investigated: moldboard plow followed by disk-harrow, strip-tillage, and para-tillage. Six herbicides treatments were investigated that were applied preemergence, postemergence to 4-leaf cotton, postemergence to 8-leaf cotton, and/or postemergence-directed. Herbicide applied included diuron, fluometuron, glyphosate, and S-metolachlor. Initial results indicate that moldboard plow followed by disk-harrowing and para-tillage reduced tropical spiderwort biomass compared to strip-tillage. Also, treatments that contained diuron, fluometuron, and/or S-metolachlor provided better control of tropical spiderwort than glyphosate-only treatments.