Field evaluation for Fusarium wilt (Fusarium oxysporum f.sp. vasinfectum (FOV) Atk. Sny & Hans) race 1 in typically sandy soil with interaction with root-knot nematodes (Meloidogyne incognita) was performed for four transgenic lines of the Upland cotton (Gossypium hirsutum L.) expressing the antifungal peptide D4E1, a transgenic control entry with the GUS marker gene and the original non-transgenic variety (Coker 312), along with commercial Acala (G. hirsutum) and Pima (G. barbadense) cultivars. Entries were planted in a randomized complete block design with four replications on 10 feet long plots. Plant survival rate, foliage damage symptoms, vascular root staining, presence of root-knot, and agronomic data will be presented for these entries. We hope to identify potentially new resistant germplasm to FOV and possibly other diseases for release in the near future.