The moisture content of seed cotton when it is ginned is known to substantially affect the quality of the ginned lint. Thus proper control of the seed cotton moisture content is an important part of the ginning process. Seed cotton drying has received significant attention but much of the seed cotton reaching the gin is drier than optimum for ginning. In this study 26 bales of DP444BR, 25 bales of DP555BR, and 9 bales of ST4892BR were ginned using different moisture treatments, including spraying a mist of water on the seed cotton in the conveyer distributor. The moisture application resulted in small but statistically significant increases in lint moisture content for samples taken behind the gin stand. The AFIS length properties were better for fiber from treatments resulting in higher moisture content. Spraying water on the low moisture content seed cotton ahead of the gin stand resulted in a statistically significant improvement in AFIS fiber length properties regardless of the cotton variety.