A field study was conducted to evaluate the effect of pelleted poultry manure and inorganic N fertilizer on cotton grwoth, seedcotton yield, nutrient uptake, and petiole nutrients in a representative cotton producing soil of Mississippi Delta Region of Arkansas. Inorganic N and pelleted poultry litter each were applied at the rates to supply 0, 30, 60, 90, 120, and 150 lb/acre of total N. Each treatment was replicated four times. Petiole samples were collected from two weeks before the bloom until the end of the fourth week of the bloom and analyzed for nutrinets. Samples of the above ground portion of plants were collected before defoliation and partioned into petiole, stems, leaves, fruits and analyzed for nutrients. Cotton growth was monitored by COTMAN monitoring tool. At harvest seedcotton yield was measured. Soil samples were collected from the top six inches after cotton harvest. The data will be used to evaluate the effect of inorganic N and pelleted poultry litter on cotton growth, seedcotton yield, nutrient uptake and soil properties.