A collection of eighty-two germplasm lines and varieties, officially released from the Pee Dee Cotton Genetics program, was included in this study to represent the life of the Pee Dee program. The eighty-two lines, along with two to six current check cultivars, were evaluated for agronomic and fiber quality traits in replicated, multi-location field trials. Data collected from this experiment will provide information on the performance of specific Pee Dee lines as compared to currently grown check cultivars. Pee Dee lines will be categorized and grouped on the basis of agronomic and fiber quality performance. Ultimately, the information within this dataset will allow for the selection of specific Pee Dee lines for specific breeding objectives.