Friday, 6 January 2006 - 8:15 AM

An Intraspecific SSR Linkage Map of Cotton

Osman A. Gutierrez1, Jixiang Wu2, Johnie N. Jenkins1, Jack C. McCarty1, Dwaine A. Raska3, and David M. Stelly4. (1) USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762, (2) Mississippi State University, Dept. of Plant & Soil Sciences, P.O.Box 5367, Mississippi State, MS 39762, (3) Texas A&M University, Department of Soil & Crop Sciences, College Station, TX 77843-2474, (4) Texa A&M University, Department of Soil and Crop Sciences, College Station, TX 77843

Most of the linkage maps that have been assembled in cotton are of interspecific nature, i.e. Upland cotton X Pima cotton (Gossypium hirsutum L. x Gossypium barbadense L.) The restriction to interspecific maps has been due to low polymorphism within the Upland cotton genotypes and insufficient numbers of SSR markers have been responsible for this situation. An intraspecific Gossypium hirsutum L. linkage map was constructed using 188 recombinant inbred lines (RIL) that were developed by single hill (bulked progeny row) procedure from the cross of HS-46 X MARCABUCAG8US-1-88. The 188 RIL were genotyped with 943 Simple Sequence Repeat (SSR) markers (BNL, CIR, JESPR and CM). A total of 211 markers (22%) were found to be polymorphic between the parents. In addition, chromosomal locations of the SSR markers were assigned using aneuploid lines. The resulting intraspecific linkage map will be presented.


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See more of The Beltwide Cotton Conferences, January 3-6 2006