A series of studies were conducted to evaluate glyphosate translocation patterns as influenced by plant growth regulator application(s) and time. The first objective examined the influence of plant growth regulator application (none or mepiquat chloride) and time. The second objective examined various plant growth regulator applications (none, mepiquat chloride, and mepiquat pentaborate) and 14C-glyphosate placement on the cotton plant. Absorption and translocation patterns were determined for both objectives. Plants were dissected by node, positions within nodes, and parts within positions. Fresh and dry weights of each part were recorded. Leaf area was also recorded.