Past research has shown that lint moisture during gin processing heavily influences the final quality of the ginned fiber. This study quantified the differences in the fiber characteristics and turnout from four commonly grown upland cotton varieties over four lint moisture regimes. The average moisture contents achieved were 4.0, 4.8, 5.6, and 6.2% (wet basis). High Volume Instrument analysis revealed that ginning at the higher lint moisture contents generally improved the fiber length and uniformity, but also increased the trash content in the ginned lint. The highest turnout of 37.7% occurred while ginning at a moisture content of 5.6%. Considering both fiber quality and turnout, ginning with lint moisture near 5.6% yielded the highest return across all four varieties, averaging about $274 per 1300 lbs of raw seed cotton. This value was about $8 more than the return from the fiber ginned at 4.0%, which was significant, and the returns from ginning at 4.8 and 6.2% were not significantly different from ginning at 5.6%.