Studies were initiated during the summers of 2004 and 2005 in Alexandria, LA to determine the effect of plant density (65,000 – 21,000 pl/A) on cotton response (growth rate, maturation, and yield) to plant growth regulator applications. The cotton variety Delta and Pine Land 'DP 555 BG/RR' was planted using a John Deere vacuum planter and hand thinned at the two leaf stage to the desired population. Plant growth regulator applications were made with a John Deere 6000 high-cycle sprayer at the 12 node, early bloom, and early bloom + two weeks stages of development. Plant height and number of nodes was recorded from early June through late-August, node above white flower documented weekly at the end of the bloom period, and yield and fiber properties analyzed to determine differences between treatments.